Could be multiple issues really:
1. Looks like your first layer height is too high
2. Temperature can cause extrusion issues
3. Can bump up extrusion in your software settings.
Sites like https://all3dp.com/2/under-extrusion-3d-printing-all-you-need-to-know/ tend to have good info on this.
I cant speak to why your test print had different results unless it has specific g-code tied to it. Also, I would check if your test print was repeatable. (ie if something was jostled out of whack etc in between prints)
What software/slicer are you using?